gene_expression

qPCR and PCR

Polymerase chain reaction (PCR) is a core and widely used laboratory method. An enhancement of this method, qPCR (quantitative PCR) measures the amplification of DNA in real time and not at the end of cycling like conventional PCR. Together these applications have contributed to significant advances in gene expression, genotyping, cloning, and other applications.

Central to PCR experimental success is the design and high quality synthesis of primers or probes. Primers and probes that are prematurely truncated, contain incorrect base sequences, or include carryover contaminants from manufacturing processes can impede accurate and efficient PCR.

Over several decades, we have refined a proprietary platform that improved nucleotide coupling efficiency during oligo synthesis. Subsequent purification steps ensure a high quality primer or probe for consistent performance. All oligos are QC tested in-house by ESI mass spectroscopy to ensure an accurate, full length, final product. Moreover, years of experience with nucleotide chemistries and thermodynamics has been incorporated into advanced algorithms powering our easy-to-use sequence design tools.

Gene expression »

Choose from probe- or intercalating dye–based qPCR options across a flexible, user-defined product offering. PrimeTime® products for gene expression are created to work well together, but can also be ordered and used separately. This modular system includes predesigned or custom assays, custom probes, master mix, and design tools.

Genotyping »

Use the rhAmp™ SNP Genotyping System as a fully integrated, high performing solution for out-of-the-box convenience. For user-defined methods, order custom probes with modifications that increase Tm enable designs within difficult sequences and with selective target identification.

Custom probes »

Select from a broad catalog of custom, fluorescent probe options. We offer several probe types, including 5′ nuclease hydrolysis probes [with or without locked nucleic acid (LNA) bases], double-quenched hydrolysis probes (with a second ZEN™ or TAO™ Quencher), or Molecular Beacon probes. Probes may be ordered with numerous dye-quencher combinations and formatting options. GMP manufactured probes are also available.

Custom primers »

Rely on us to provide quality primers needed for consistent results. Our primers can be used as part of a larger product solution or ordered as standalone core reagents. Design and ordering is easy using our free, online tools.

Master mixes, enzymes, and reagents »

Select easy-to-use pre-formulated mixes to complete your gene expression or genotyping experiment. Core reagents (RNase H enzyme, buffers, and solutions) are also available for more individualized needs.

Inventoried oligos »

Choose from a wide variety of ReadyMade™ oligonucleotides for sample preparation, PCR, and gene expression analysis. Inventoried oligos are ready to ship when your order is received and include oligo dT, random hexamers, and GAPDH primers.