qPCR and PCR

Polymerase chain reaction (PCR) is a core and widely used laboratory method. An enhancement of this method, qPCR (quantitative PCR) measures the amplification of DNA in real time and not at the end of cycling like conventional PCR. Together these applications have contributed to significant advances in gene expression, genotyping, cloning, and other applications.

Central to PCR experimental success is the design and high quality synthesis of primers or probes. Primers and probes that are prematurely truncated, contain incorrect base sequences, or include carryover contaminants from manufacturing processes can impede accurate and efficient PCR.

Over several decades, we have refined a proprietary platform that improved nucleotide coupling efficiency during oligo synthesis. Subsequent purification steps ensure a high quality primer or probe for consistent performance. All oligos are QC tested in-house by ESI mass spectroscopy to ensure an accurate, full length, final product. Moreover, years of experience with nucleotide chemistries and thermodynamics has been incorporated into advanced algorithms powering our easy-to-use sequence design tools.