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  1. Products
  2. qPCR & PCR
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qPCR and PCR

IDT offers a variety of qPCR and PCR products to meet your research needs. Order predesigned and custom probes and primers, master mixes, and ready-to-use qPCR solutions.

Gene expression

Choose from probe- or intercalating dye-based qPCR options across a flexible, user-defined product offering. PrimeTime™ products offer a full workflow solution, but components can also be ordered and used separately. This modular system includes predesigned or custom assays, custom probes, master mix, and design tools.

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Genotyping

The rhAmp™ SNP Genotyping System is a fully integrated genotyping solution that includes an extensive collection of predesigned primers and probes, a custom design tool, optimized reagent mixes, and optional synthetic control templates. The PACE™ system (3CR Bioscience) offers a cost-effective, flexible solution for high-throughput applications. For user-defined methods, order custom probes such as locked nucleic acid probes and assays with modifications that increase Tm. Locked nucleic acid modifications can help enable probe designs within challenging sequences and with selective target identification.

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Custom probes

Select from a broad catalog of custom fluorescent probe options, including Affinity Plus™ qPCR Probes with locked nucleic acids, double-quenched hydrolysis probes, and molecular beacon probes. Each of these probe types may be customized with numerous dye-quencher combinations and formatting options.

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Custom primers

Use our quality primers as part of a larger product solution, or order as stand-alone core reagents. Design and ordering is easy using our free, online tools.

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Master mixes, enzymes, and reagents

Select easy-to-use, preformulated one-step and two-step mixes, including the PrimeTime One-Step 4X Broad-Range Master Mix and rhAmp Genotyping Master Mix, or purchase stand-alone core reagents to complete your experiments.

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Inventoried oligos

Choose from a wide variety of ReadyMade™ oligonucleotides for sample preparation, PCR, and gene expression analysis that are ready to ship when your order is received.

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GMP products and services

Fast, easy, and clear process to determine the best products and services to meet your specific needs for more rigorous quality requirements.

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Overview

Polymerase chain reaction (PCR) is a core and widely used laboratory method. An enhancement of this method, qPCR (quantitative PCR, also known as real-time PCR) measures the amplification of DNA in real time and not at the end of cycling like conventional PCR. Together these applications have contributed to significant advances in gene expression, genotyping, cloning, and other applications. Central to PCR experimental success is the design and high-quality synthesis of primers or probes. Primers and probes that are prematurely truncated, contain incorrect base sequences, or include carryover contaminants from manufacturing processes can impede accurate and efficient PCR.

Over several decades, we have refined a proprietary platform that improved nucleotide coupling efficiency during oligo synthesis. Subsequent purification steps produce a high-quality primer or probe. All oligos are QC tested in-house by ESI mass spectroscopy* to help ensure an accurate, full-length, final product. Moreover, years of experience with nucleotide chemistry and thermodynamics have been incorporated into advanced algorithms powering our easy-to-use sequence design tools.

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

GMP refers to products manufactured under ISO 13485: 2016 QMS. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations for their legal marketing.

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.

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