High performance liquid chromatography (HPLC) purification, a form of column chromatography, can be performed in one of two ways. Reverse-phase (RP) HPLC separates oligos based on their affinity for particular solvents, while ion-exchange (IE) HPLC separates
oligonucleotides based on their charge. The type of HPLC we use is dependent on the sequence and molecular characteristics of the oligo. HPLC-purified oligos experience an unavoidable loss of mass, but this is offset by the dramatic increase in purity. To learn more about our purification services, click here.
Rapid HPLC Oligos are purified using the same equipment and protocols as standard HPLC purification. The resulting purity is >85% full-length product. All oligos are quality control checked by electrospray ionization—mass spectrometry (ESI-MS),
with traces available for free on our website. If you place your order before the day’s deadline (below), your Rapid HPLC Oligos will be shipped on the next business day.