Available in convenient sizes:
|Scale (pmol/oligo)||Number of oligos per pool||Oligo length (bases)|
|1||100 to 20,000||40 to 350|
|10||10 to 2000||40 to 350|
|50||2 to 384||40 to 350|
oPools Oligo Pools are single-stranded DNA sequences that are used for CRISPR library construction, primer pools for multiplex PCR, gene construction, data storage, and FISH analysis.
Proprietary DNA synthesis equipment permits rapid, high-quality synthesis of nucleic acids. This platform is the same proprietary synthesis platform used to make IDT Ultramer™ DNA Oligos. It uses an "extra rich" synthesis cycle for long oligos. Along with this refined synthesis cycle, oPools DNA Oligos use a unique solid support specifically optimized to synthesize high-quality oligos up to 350 bases in length.
|Scale (number of oligos)|
|Mixed bases ||N = A, C, G, and T|
K = G and T
• Limited to 9 mixed bases per oligo
|QC and quantification||None|
|Shipping container||2 mL tube|
Figure 1. High coupling efficiency results in a greater amount of full-length product than other suppliers. oPools Oligo Pools are manufactured using the same proprietary synthesis platform as Ultramer™ DNA Oligos and offer higher coupling efficiencies than industry standards. The amount of full-length oligo is calculated using coupling efficiency. As seen in the graph, coupling efficiency of oPools Oligo Pools (99.6%) allows for longer oligos to be synthesized compared to coupling efficiency of 98.5% from three leading competitors.
Figure 2. Proprietary DNA synthesis results in an even yield distribution of oPools Oligo Pools. This even yield distribution is shown here as a function of % difference from the mean. The standard deviation observed across ½ million sequences is less than 23% of the mean, demonstrating a high level of uniform sequence representation.
Dropout rate refers to the likelihood that any individual sequence is not present in the final pool. This rate can vary by length and sequence complexity. The average dropout rate for oPools oligos across all length ranges was 0.8% based on a sample size of over 900,000 oligos.