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Whole transcriptome sequencing

In today’s competitive genomics landscape, RNA sequencing will help you quickly achieve your desired results. Our xGen RNA sequencing product offerings cover a wide range of diverse inputs while maintaining predictable outputs, efficiencies, and coverage metrics.

xGen™ NGS—made to differentiate.

Overview

  • Fast and cost-efficient workflow—curated for RNA sequencing
  • Compatible with a range of input types and quantities—prepare RNA-seq libraries from a wide range of sample types and input quantities
  • Powered by Adaptase™ technology—for adapter tailing and ligation to 1st strand DNA and does not require 2nd strand DNA
  • Flexible and customizable—easily combine a hybridization capture workflow for targeted RNA-seq
  • Combine the xGen Normalase™ Module with your RNA-seq workflow—for fast and easy library balance and pooling
  • Automation-friendly RNA-seq workflow

RNA sequencing (RNA-seq) is a next generation sequencing method for analyzing the transcriptome. The xGen RNA Library Prep Kit is a fast NGS transcriptomics research workflow.

This stranded RNA-seq workflow uses Adaptase™ technology which involves 3’ tailing and ligation of R1 Stubby Adapter to the first strand of cDNA and does not require 2nd stand cDNA synthesis and degradation, or template switching methods.

This RNA-seq workflow is compatible with upstream poly(A) selection and rRNA depletion modules. Easily combine with a hybridization capture workflow for a targeted mRNA capture sequencing study.

For high-throughput labs, the 96-reaction size group of products includes the xGen Normalase Module, a proprietary enzymatic library normalization kit for multiplexing. The method eliminates the need for individual sample quantification followed by manual equimolar pooling. Instead, the fragments are indexed by PCR using the included plate of xGen Normalase UDI Primer Pairs followed by the xGen Normalase Module. Final normalized library pools are then ready for Illumina-based multiplex sequencing.

Icons Library_White Outline_85x85_Extraction

Extraction

Icons Library_White Outline_85x85_mRNA enrichment

mRNA enrichment

Icons Library_White Outline_85x85_Normalase

Normalization

xGen Normalase Module

Icons Library_White Outline_85x85_Sequence and analyze

Sequencing & analysis

IDT ALIGN Program

Method Data

Figure 1. Normalase technology enables consistent pooling of RNA-seq libraries without compromising transcriptomic data quality. The IDT xGen RNA Library Prep Kit and xGen Broad-Range RNA Library Prep Kit were used to create whole transcriptome libraries from universal human total RNA samples (Thermo Fisher Scientific; Cat. No. QS0639) that were either ribodepleted or poly(A)-selected upstream of library construction. Libraries were indexed using xGen Normalase CDI primers and amplified with the number of PCR cycles recommended in the respective protocols. Library pools were then normalized using the xGen Normalase Module and sequenced to determine the percent reads identified for each index. Respective sequencing platforms and the number of pooled libraries are indicated in the figure.

Ordering

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.

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