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RNA oligos

Specificity you need for your functional genomics research

All single-stranded and duplexed RNA sequences are produced using proprietary technology that delivers industry-leading RNA quality. Our specialized synthesis platforms allow us to produce the highest fidelity guides for CRISPR, duplexes for RNAi, template switching oligos for NGS, microRNAs, aptamers, and custom RNA oligos for other applications.

  • Tailor RNA oligos to your application by selecting from chimeric DNA bases, modified RNA bases, and a wide variety of modifications
  • Conduct your experiment sooner with fast turnaround times for desalted and HPLC-purified RNA oligos
  • Have complete confidence in oligos that are quality controlled by ESI-mass spectrometry*
  • For your convenience every RNA oligo is deprotected and shipped ready to use

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.


Single-stranded RNA

Shipped dry.


Duplexed RNA

2 oligos, annealed and delivered in a single tube. Shipped dry.


Annealing fees will be applied to duplexed oligos (per duplex), as follows:

100 nmol:


250 nmol:


1 µmol:


Single-stranded RNA

Shipped dry or resuspended to your specifications. A minimum of 24 or 96 oligos required for 96- or 384-well plates, respectively.


RNA oligos are short, single- or double-stranded synthetic RNA sequences that can be used in nearly any RNA-specific molecular biology application. Using the Oligo Entry ordering tool, you can design your sequences to contain unmodified RNA bases, 2′-O-methyl RNA bases, or chimeric DNA bases. You can select from hundreds of modifications to suit your specific research needs. (2′-O-methyl RNA is a useful RNA analog that is resistant to degradation by single-stranded ribonucleases.)

Every RNA Oligo you receive is deprotected and desalted to remove small molecule impurities. In addition, your oligos will be sequence-verified via proprietary ESI mass spectrometry methods*, and quantified twice by UV spectrophotometry to provide accurate yield measurements.

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

Using the RNA Oligo Entry tool

  • To enter individual RNA bases, insert "r" before the base (rA, rU, rC, rG)
  • To enter individual 2′-O-Methyl RNA bases, insert "m" before the base (mA, mU, rC, rG)
  • To quickly convert an entire oligo to RNA, enter your sequence as DNA using A, T, C, G and then click “Convert to “RNA”. All bases in your sequence will be converted to RNA (rA, rU, rC, rG)

IDT’s long-standing reputation as a leader and pioneer in custom oligo manufacturing is largely due to our vertically integrated proprietary synthesis platforms. Nearly every component of our manufacturing process is designed and developed in-house, including specialized synthesizers that can accommodate the most demanding oligo requests, and high-throughput automation systems that ensure the fastest turnaround times possible. We do not rely on third-party manufacturers for any of the machines or chemical reagents used for synthesis, allowing us to easily monitor and refine instruments and reagents with ease. Our ability to control these variables allows us to produce oligos that are unmatched in quality and consistency.

The inherent structure of RNA makes it more difficult to manufacture than DNA. However, our expertise in nucleic acid synthesis allows us to consistently achieve the highest coupling efficiencies in the industry, meaning you will always receive RNA oligos with the yield and purity that your project requires.

Frequently asked questions

OligoAnalyzer® tool

Identify oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches

UNAFold tool

Perform secondary structure analysis

Dilution Calculator

Find the volume needed to dilute your oligo stock to a lower concentration

Resuspension Calculator

Find the volume needed to resuspend a dry oligo to a desired concentration

The following services represent our most common offerings. If your project requires a service not listed here, contact, and our experts will work with you to accommodate your request.


For more demanding RNA applications, additional purification by RNase-Free HPLC purification will significantly improve oligo performance. All purified oligos up to 60 bases receive a purity guarantee following quality control by capillary electrophoresis. Learn more »



Diverse application requirements and individual researcher preferences necessitate oligos that can be delivered in unique formats. You can have your oligos prepared to your specific requirements. Learn more »

For information on available services, email

Custom quality control

In addition to the free ESI mass spectrometry analysis that is provided for your oligos*, your project may require one of the more advanced analytical services listed below.

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.


Large-scale synthesis

Custom RNA oligos can be ordered in bulk with the same high purity, competitive pricing, and fast turnaround times you expect from IDT. To learn more about our large-scale synthesis capabilities, click here.


If you require oligos that are approved for use in molecular diagnostic applications, or if you are interested in our third-party manufacturing services, please click here.