For demanding applications such as multiplex PCR, cloning, mutagenesis or antisense/RNAi methods, additional purification can significantly improve oligonucleotide performance. Purified oligos up to 60 bases that are provided with a purity guarantee receive
QC by capillary electrophoresis (CE). The traces are available online in your account order history.
Purification services offered include PAGE and various types of HPLC. Purified, unmodified oligos 20–50 bases in length are provided with the guaranteed yields (Table 1).*
Table 1. Yield guarantees for unmodified oligos of 20–50 bases, after purification.
|Purification type||100 nmol||250 nmol||1 µmol||5 µmol||10 µmol|
|PAGE||1 OD||2 OD||10 OD||50 OD||100 OD|
|HPLC||1 OD||4 OD||20 OD||100 OD||200 OD|
|IE-HPLC||1 OD||4 OD||20 ODs||100 OD||200 OD|
|RNase-Free HPLC||1 OD||4 OD||20 ODs||100 OD||200 OD|
|Dual HPLC||1 OD||2 OD||10 ODs||50 OD||100 OD|
|Dual PAGE & HPLC||0.5 OD||1 OD||5 ODs||25 OD||50 OD|