IDT SciTools® Web Tools

These software applications determine the properties of any oligo sequence entered, as well as facilitate the intelligent design of assay conditions, all at the click of a button. The suite is easy to use and makes it simple to add each optimized oligonucleotide to an ongoing order list ready for purchase.

Oligo Analysis Tools

OligoAnalyzer® Tool
Identifies oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches
UNAFold Tool
Secondary structure analysis
Dilution Calculator
Find the volume needed to dilute your oligo stock to a lower concentration
Resuspension Calculator
Find the volume needed to resuspend a dry oligo to a desired concentration

qPCR and PCR Amplification

PrimeTime® Predesigned qPCR Assay Database
Selects qPCR primers and probe sequences for human, mouse, and rat assays based on up-to-date sequence data
PrimerQuest® Tool
Allows highly customized designs for PCR primers and qPCR assays
RealTime PCR Tool
Selects custom qPCR assays for any species

Gene Regulation and Knockdown

Predesigned DsiRNA Selection Tool
Selects DsiRNA Duplexes and TriFECTa® Screening Kits for your sequence
RNAi Design Tool
Generates duplex siRNA sequences for RNAi applications

Gene Design

Codon Optimization Tool
Automates codon optimization of a gene to the species of your choice

Sequencing and miRNA

454 Fusion Primers
Designs primers for next generation sequencing on 454 GS GLX Standard or Titanium Platforms
miRNA Detection Kit Selection Tool
Identifies the IDT mirFire™ Probe or Labeling Kit needed to label a specific miRNA
Please use following format when citing SciTools:

PrimerQuest® program, IDT, Coralville, USA. Retrieved 12 December, 2012.