Researchers often look for genetic variations to better understand phenotypic traits, such as plant fruit production or human disease incidence. One individual’s genome may differ from others in numerous ways, including single nucleotide polymorphisms
(SNPs), insertions or deletions (indels), or copy number variations (CNV).
Polymerase chain reaction (PCR) is a commonly used genotyping technique. Often employing a primer-pair and target-specific fluorescent probe, quantitative PCR (qPCR) can be a sensitive and specific way to detect SNPs. IDT offers a complete SNP-typing
solution with predesigned assays, as well as complementary easy- and ready-to-use reagent mixes. For other applications, modified probes are available that can be incorporated into custom qPCR assays.