Figure 6. Variability in size of genomes, input amount, and GC content do not influence results in a metagenomics analysis. (A) Libraries were prepared using the MSA-1000™ microbiome standard (ATTC, an artificial
microbial community of 10 strains), Lotus kit, full-length adapters, and PCR amplification with P5 and P7 primers. (B) Libraries were prepared with 5 ng of MSA-1000 gDNA. Normalized genome coverage for 3 representative strains with varying
GC% content is shown as colored lines The number of 100 bp regions at each GC% is shown as histograms.