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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How do 5' phosphorylated primers affect PCR?

Primers that are 5' phosphorylated do not affect PCR since primer extension occurs from the 3' end of the primer. 5' phosphates on each strand of the amplicon are reqiured for ligation of the PCR product into a vector.