Flexible tool input formats and advanced parameters help you create custom, targeted sequencing panels optimized for your targets:
Custom rhAmpSeq Panels have been designed and tested in a wide range of animal and plant species and diverse targeted NGS applications, including:
For the rhAmpSeq Sample ID Panel, performance has been optimized to achieve >95% mapped reads, on-target rate, and coverage uniformity (% reads >0.2X of the mean coverage) across multiple sample types.
For Custom rhAmpSeq Panels, we typically observe >90% mapped reads, >90% on-target rates, and >85% coverage uniformity for human panels across panel sizes up to 3000-plex using highly purified reference gDNA. Results may vary based on several factors, including species and availability of reference genome, input targets, panel size, and sample type/quality.
After years of using traditional amplicon sequencing in grapevine, we found that rhAmpSeq’s specificity and evenness of read depth across sites and samples enabled us to multiplex to much higher levels. IDT provided agricultural pricing that enables us to apply 2000 core genome rhAmpSeq haplotype markers to genetic mapping and marker-assisted selection for grape scientists worldwide. ”