Icons_Sky_60x60_Tools

rhAmpSeq Design Tool

Deep, targeted amplicon sequencing with highly multiplexed panels for sequencing on Illumina platforms.

  • Generate sequencing panels for a wide range of plant and animal species
  • Minimize primer dimers and maximize multiplexing capability
  • Efficiently analyze on- and off-target CRISPR edit sites
  • Multiplex up to 5000-plex in a single pool

Research-friendly UX

Flexible tool input formats and advanced parameters help you create custom, targeted sequencing panels optimized for your targets:

  • Choose BED or FASTA inputs
  • Optimize for SNP hotspot or CRISPR InDel screening
  • Name & save designs for easy reuse and reordering
amplicon sequencing tool

Generating NGS-ready amplicon libraries just got easier

Easy to use sequencing design tool
Design for hotspot target SNPs, indels, and more
Icons_Sky_85x85_Speed
Obtain panel designs within 1 hour for most applications*
Easy to use sequencing design tool
Accelerate discovery with quicker, easier ordering
* Larger and more complex panels of 1000+ targets may require a couple days of processing time

rhAmpSeq Design Tool tutorial video

A tutorial video that will guide you through the process of using the rhAmpSeq Design Tool, and several of its features.

Tailored to your research

Custom rhAmpSeq Panels have been designed and tested in a wide range of animal and plant species and diverse targeted NGS applications, including:

  • Marker-assisted selection and genomic selection in agricultural genetics and breeding
  • Confirmation of on-target and off-target CRISPR gene editing experiments
  • Hotspot panels for oncology, rare and inherited diseases, and other disease research

Resources

Frequently asked questions

What is the sequencing performance of the rhAmpSeq system?

Mar 6, 2019, 13:00 PM

For the rhAmpSeq Sample ID Panel, performance has been optimized to achieve >95% mapped reads, on-target rate, and coverage uniformity (% reads >0.2X of the mean coverage) across multiple sample types.

For Custom rhAmpSeq Panels, we typically observe >90% mapped reads, >90% on-target rates, and >85% coverage uniformity for human panels across panel sizes up to 3000-plex using highly purified reference gDNA. Results may vary based on several factors, including species and availability of reference genome, input targets, panel size, and sample type/quality.

Tags :
  • genotyping
  • ngs
Categories :
  • FAQs
  • Next generation sequencing
  • rhAmpSeq custom panels
  • rhAmpSeq index primers
  • rhAmpSeq Library Kit
  • rhAmpSeq Sample ID Panel
Load more comments
comment-avatar

Brochures and flyers

After years of using traditional amplicon sequencing in grapevine, we found that rhAmpSeq’s specificity and evenness of read depth across sites and samples enabled us to multiplex to much higher levels. IDT provided agricultural pricing that enables us to apply 2000 core genome rhAmpSeq haplotype markers to genetic mapping and marker-assisted selection for grape scientists worldwide.

Bruce Reisch
Professor of Grapevine Genetics and Family Genealogist
Cornell University