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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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When preparing the qPCR reaction mix should I thaw or keep reagents (such as the Primetime™ Gene Expression Master Mix) on ice?
It is good practice, but not necessary to thaw or keep reagents on ice. We see no difference in results between reaction mixes prepared with the PrimeTime™ Gene Expression Master Mix that were used directly after thawing vs. those kept at room temperature for up to 24 hours.
PrimeTime Gene Expression Master Mix also shows exceptional temperature stability, even after a heat stress test (up to 8 hr at 55°C). Review the data in our white paper, Ambient shipping of PrimeTime Gene Expression Master Mix.
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qPCR & PCR*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.