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It is good practice, but not necessary to thaw or keep reagents on ice. We see no difference in results between reaction mixes prepared with the PrimeTime™ Gene Expression Master Mix that were used directly after thawing vs. those kept at room temperature for up to 24 hours.
PrimeTime Gene Expression Master Mix also shows exceptional temperature stability, even after a heat stress test (up to 8 hr at 55°C). Review the data in our white paper, Ambient shipping of PrimeTime Gene Expression Master Mix.
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