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Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
What is the minimum number of bases that can be annealed?
You can anneal an oligo of any size to its target, but longer sequences lead to more stable duplexes.
A minimum of 10 bases is needed for a PCR primer to bind and remain annealed to its target long enough for extension to occur. This may be a good minimum threshold to consider if you plan to anneal DNA.