Get Help Sign In

Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Should I amplify my gBlocks™ HiFi Gene Fragments when I receive them?

Most applications such as restriction-digest, blunt-end, and Gibson cloning can be performed with the material provided by IDT, and PCR amplification should not be necessary. If the purpose of the amplification is to add flanking bases to complete a cloning reaction, we strongly recommend ordering the gBlocks™ HiFi Gene Fragment (IDT) with the required bases rather than adding them afterwards using PCR.

If you must amplify your gBlocks HiFi Gene Fragment, use the minimum amount of starting template required by your PCR kit protocol, limit the cycle number to 10–12, and use a high-fidelity polymerase.


*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.