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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How much Alt-R® Cas9 Electroporation Enhancer should I use during delivery of the ribonucleoprotein (RNP) complex?

We see dose response curves that vary for different guide sequences, depending on the potency of the guide sequence. We recommend titrating the amount of RNP for each cell type of interest.

Start with a 1:1 ratio of enhancer:RNP and optimize using a titration gradient of the RNP. For the Amaxa® Nucleofector® System (Lonza), we recommend 4 µM of Alt-R® Cas9 Electroporation Enhancer, and for the Neon® System (Thermo Fisher Scientific), we recommend 1.8 µM of Alt-R Cas9 Electroporation Enhancer.

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.