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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How many reactions will I be able to do with 25 nmol PCR primers?

Most PCR reactions use 0.1−0.5 μM primer.Assuming a maximum concentration of 0.5 μM and a reaction volume of 20 μL, each reaction will require 10 pmol oligonucleotide primer.

For a typical 25mer oligonucleotide, 1 OD is equivalent to approximately 4 nmol, or 4000 pmol. Guaranteed yield for a 20 base PCR primer on the 25 nmol scale is 12 nmol. Therefore, with even the minimum yield from a 25 nmol synthesis, you should be able to perform 1200 PCR reactions.

In summary: Minimum guarantee for a 20mer ≈ 12 nmol = 12,000 pmol = 1200 reactions.

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