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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How does Alt-R® S.p. HiFi Cas9 Nuclease V3 differ from the standard Alt-R S.p. Cas9 Nuclease V3?

Alt-R S.p. HiFi Cas9 Nuclease V3 was developed to reduce off-target cutting events, with similar high, on-target activity as the Alt-R S.p. Cas9 Nuclease V3.

Alt-R HiFi Cas9 nuclease can be substituted for Alt-R Cas9 nuclease in experiments that are sensitive to off-target cutting events. We recommend testing a dose-response curve using Alt-R HiFi Cas9 or other Cas9 proteins to determine the optimal dosage.

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.