Yes, IDTE Buffer is nuclease-free.
We test for both DNases and RNases using our DNaseAlert and RNaseAlert Kits.
http://eu.idtdna.com/pages/support/faqs/is-idte-buffer-rnase-free-Yes, IDTE Buffer is nuclease-free.
We test for both DNases and RNases using our DNaseAlert and RNaseAlert Kits.
http://eu.idtdna.com/pages/support/faqs/is-idte-buffer-rnase-free-No. 1X TE buffer is typically 10 mM Tris, 1 mM EDTA, while IDTE (1X TE solution) is 10 mM Tris, 0.1 mM EDTA. The additional EDTA in 1X TE buffer can...
http://eu.idtdna.com/pages/support/faqs/can-1x-te-buffer-be-used-instead-of-idte-buffer-in-rhampseq-experimentsNo. 1X TE buffer is typically 10 mM Tris, 1 mM EDTA, while IDTE (1X TE solution) is 10 mM Tris, 0.1 mM EDTA. The additional EDTA in 1X TE buffer can...
http://eu.idtdna.com/pages/support/faqs/can-1x-te-buffer-be-used-instead-of-idte-buffer-in-rhampseq-experimentsWe recommend using a buffer made of 10 mM Tris, 0.1 mM EDTA, and 100 mM NaCl, pH 8.0 (such as the IDT NGS Adapter Buffer, catalog # 10006743) to dilute the xGen Stubby Adapter for Element.
Keeping the adapters duplexed requires a buffer with a ...
http://eu.idtdna.com/pages/support/faqs/what-buffer-should-i-use-to-dilute-the-xgen-stubby-adapter-for-elementWe recommend using a buffer made of 10 mM Tris, 0.1 mM EDTA, and 100 mM NaCl, pH 8.0 (such as the IDT NGS Adapter Buffer, Cat. No. 10006743) to dilute the xGen Adapters...
http://eu.idtdna.com/pages/support/faqs/what-buffer-should-be-used-to-dilute-the-xgen-stubby-adapterWe recommend using a buffer made of 10 mM Tris, 0.1 mM EDTA, and 100 mM NaCl, pH 8.0 (such as the IDT NGS Adapter Buffer, Cat. No. 10006743) to dilute the xGen Stubby Adapter for DNBSEQ. Keeping the adapters duplexed requires a buffer with a high salt ...
http://eu.idtdna.com/pages/support/faqs/what-buffer-should-i-use-to-dilute-the-xgen-stubby-adapter-for-dnbseqWe recommend using a buffer made of 10 mM Tris, 0.1 mM EDTA, and 100 mM NaCl, pH 8.0 (such as the IDT NGS Adapter Buffer, Cat. No. 10006743) to dilute the xGen™ Stubby Adapter, xGen™ PCR-Free Adapters for Ultima, and xGen™ HybCap Adap...
http://eu.idtdna.com/pages/support/faqs/what-buffer-should-i-use-to-dilute-the-xgen-stubby-adapter-xgen-pcr-free-adapters-for-ultima-and-xgen-hybcap-adapters-for-ultimaAll Cas12a variants are provided as a 10 mg/mL solution and, therefore, resuspension is not required.
http://eu.idtdna.com/pages/support/faqs/what-buffer-should-i-use-to-resuspend-the-cpf1-proteinAll Alt-R™ Cas9 nucleases and nickases are provided in solution at 10 mg/mL, so resuspension is not necessary.
http://eu.idtdna.com/pages/support/faqs/what-buffer-should-i-use-to-resuspend-the-cas9-proteinThe Element Biosciences Trinity flow cell and reagents allow users to capture biotinylated baits and bound targets on-instrument and thus do not require streptavidin-coated magnetic beads or additional wash buffers.
http://eu.idtdna.com/pages/support/faqs/why-does-my-xgen-exome-sequencing-kit-trinity-for-element-only-contain-xgen-2x-hybridization-buffer-xgen-hybridization-buffer-enhancer-and-human-cot-dnaAlthough it may be possible to anneal oligos at room temperature, heating to denature the oligos and then cooling slowly to anneal the two oligos will help to ensure more efficient annealing and favor the stable duplex formation.
If you choose ...
http://eu.idtdna.com/pages/support/faqs/can-i-anneal-my-dna-oligos-at-room-temperature-and-if-so-what-buffer-should-i-use-IDT uses our own proprietary HPLC method to purify oligos in-house. A published set of conditions for an RP-HPLC protocol is:
Yes, both products are the same protein construct and the same formulation buffer to ensure equivalent performance. Specifications are very similar between the two products.
http://eu.idtdna.com/pages/support/faqs/are-the-ruo-and-gmp-products-the-sameIDT ships custom gene dry unless otherwise requested. While dry they should be stable up to 2 years. After resuspending in a high-quality, molecular-grade water or buffer, pH 7.5–8.0...
http://eu.idtdna.com/pages/support/faqs/what-are-optimum-storage-conditions-for-a-custom-gene-We find it convenient to initially make a 100 µM freezer stock, which should be thawed relatively infrequently to make more dilute aliquots for short term use. The oligo can be dissolved in TE buffer...
http://eu.idtdna.com/pages/support/faqs/how-do-i-resuspend-my-oligosAs long as you know the concentration of the monovalent cations in your solution (Na+, K+, etc.), you can use our free OligoAnalyzer™ Tool to calculate the Tm of your oligo...
http://eu.idtdna.com/pages/support/faqs/how-do-i-calculate-the-melting-temperature-of-oligos-in-salt-solutions-other-than-nacl-Under digestion is caused by either improper fragmentation time—see the CoA or product box for recommended lot-specific fragmentation time for the supported insert sizes; incomplete mixing of the master mix before or when combined with the sample...
http://eu.idtdna.com/pages/support/faqs/what-can-cause-under-fragmentation-of-dnaFollow these steps to resuspend Alt-R™ HDR Donor Blocks:
Yes. IDT offers a service called the LabReady service on standard products. With the LabReady service, we ship your oligos at a 100 µM concentration in IDTE Buffer...
http://eu.idtdna.com/pages/support/faqs/can-i-order-my-oligos-resuspended-to-a-100um-concentration-We recommend briefly centrifuging your tubes of dried oligo prior to opening them.
This will ensure that the oligo pellet is at the bottom of the tube and will not be lost when you open the cap. After adding non-DEPC treated water or buffer...
http://eu.idtdna.com/pages/support/faqs/should-i-do-anything-to-prep-my-tube-of-oligo-before-resuspending-Any charge will play a role in the Tm value for an oligonucleotide in a reaction. Thus, sodium, magnesium, and potassium concentrations each contribute to this calculation.
The IDT OligoAnalyzer™ Tool...
http://eu.idtdna.com/pages/support/faqs/should-i-only-be-concerned-about-sodium-ions-when-calculating-tm-The shelf life of an oligo is dependent on the temperature at which the oligo is stored and how the oligo is resuspended. Temperature is the more important of the 2 variables. Generally, oligos should be stored at –20°C. At this temper...
http://eu.idtdna.com/pages/support/faqs/what-is-the-shelf-life-of-my-oligoIDT ships gBlocks HiFi Gene Fragments (IDT) dry unless otherwise requested. While dry, they should be stable almost indefinitely.
A...
http://eu.idtdna.com/pages/support/faqs/what-are-optimum-storage-conditions-for-a-gblocks-hifi-gene-fragmentThe inherent chemical structure of RNA makes it less stable than DNA. RNases that degrade RNA are also more prevalent than DNases.
As RNA is a great deal more sensitive to degradation compared to DNA, for short term storage we recommend using...
http://eu.idtdna.com/pages/support/faqs/should-rna-be-stored-differently-than-dnaIDT uses our own proprietary HPLC method to purify oligos in-house. A published set of conditions for an RP-HPLC protocol is:
The Alt-R Cas9 nucleases and nickases are supplied at concentration of 10 mg/mL in 25 mM Tris-Cl, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol (v/v), pH 7.4, with the...
http://eu.idtdna.com/pages/support/faqs/what-is-the-concentration-of-the-alt-r-sup-sup-cas9-nucleases-and-nickasesThe shelf life of an oligo is dependent on the temperature at which the oligo is stored and how the oligo is resuspended. Temperature is the more important of the 2 variables. Generally, oligos should be stored at –20°C. At this temper...
http://eu.idtdna.com/pages/support/faqs/what-is-the-shelf-life-of-my-oligoYes. IDT offers a service called the LabReady service on standard products. With the LabReady service, we ship your oligos at a 100 µM concentration in IDTE Buffer...
http://eu.idtdna.com/pages/support/faqs/can-i-order-my-oligos-resuspended-to-a-100um-concentration-No, we do not recommend resuspending or storing oligos in DEPC treated water. DEPC treated water can be acidic which may cause depurination of the oligo resulting in degradation.
We recommend that oligos are stored long term at at ‒20°C. ...
http://eu.idtdna.com/pages/support/faqs/is-it-ok-to-resuspend-and-store-oligos-in-depc-treated-water-IDT ships Alt-R™ HDR Donor Blocks dry at ambient temperatures. While dry, we recommend long term storage at -20°C. After resuspending in a high quality, molecular-grade water or buffer, pH 7.5-8.0...
http://eu.idtdna.com/pages/support/faqs/what-are-optimum-storage-conditions-for-alt-r-hdr-donor-blocksNo, we do not recommend resuspending or storing oligos in DEPC treated water. DEPC treated water can be acidic which may cause depurination of the oligo resulting in degradation.
We recommend that oligos are stored long term at at ‒20°C. ...
http://eu.idtdna.com/pages/support/faqs/is-it-ok-to-resuspend-and-store-oligos-in-depc-treated-water-The stability of fluorescently labeled oligos and fluorophore function is similar to the stability of a standard oligo.
IDT recommends oligos that are to be stored long term should be stored frozen at –20°C. If the oligos are going ...
http://eu.idtdna.com/pages/support/faqs/what-is-the-stability-of-fluorescently-labeled-oligos-and-their-fluorescent-modificationsImproper fragmentation time is one cause of over fragmentation; see the CoA or the product box for the recommended lot-specific fragmentation time for supported fragment sizes.
Over digestion is another cause a...
http://eu.idtdna.com/pages/support/faqs/what-can-cause-over-fragmentation-of-dna-samplesThe amount of xGen UDI-UMI Adapters you use depends on the library prep workflow and the amount of starting sample used to perform librar...
http://eu.idtdna.com/pages/support/faqs/how-much-xgen-udi-umi-adapters-should-i-use-for-library-prepFor the modifications tested at this point, the pattern of degradation seen for modified oligos is similar to that for standard oligos. However, stability or shelf-life information has not been collected for all modifications offered.
For...
http://eu.idtdna.com/pages/support/faqs/do-modifications-affect-the-stability-or-shelf-life-of-oligosThe Alt-R CRISPR-Cas9 System provides custom CRISPR crRNA and a universal CRISPR tracrRNA, both of which are required for CRISPR-Cas9 genome editing using the Alt-...
http://eu.idtdna.com/pages/support/faqs/what-reagents-will-i-need-in-addition-to-the-alt-r-rnas-to-do-a-crispr-genome-editing-experimentWe recommend briefly centrifuging your tubes of dried oligo prior to opening them.
This will ensure that the oligo pellet is at the bottom of the tube and will not be lost when you open the cap. After adding non-DEPC treated water or buffer...
http://eu.idtdna.com/pages/support/faqs/should-i-do-anything-to-prep-my-tube-of-oligo-before-resuspending-Any charge will play a role in the Tm value for an oligonucleotide in a reaction. Thus, sodium, magnesium, and potassium concentrations each contribute to this calculation.
The IDT OligoAnalyzer™ Tool...
http://eu.idtdna.com/pages/support/faqs/should-i-only-be-concerned-about-sodium-ions-when-calculating-tm-As long as you know the concentration of the monovalent cations in your solution (Na+, K+, etc.), you can use our free OligoAnalyzer™ Tool to calculate the Tm of your oligo...
http://eu.idtdna.com/pages/support/faqs/how-do-i-calculate-the-melting-temperature-of-oligos-in-salt-solutions-other-than-nacl-The stability of fluorescently labeled oligos and fluorophore function is similar to the stability of a standard oligo.
IDT recommends oligos that are to be stored long term should be stored frozen at –20°C. If the oligos are going ...
http://eu.idtdna.com/pages/support/faqs/what-is-the-stability-of-fluorescently-labeled-oligos-and-their-fluorescent-modificationsFor long term oligo storage, temperature is the most important factor to consider. For long term storage, whether oligos are dried down, or ...
http://eu.idtdna.com/pages/support/faqs/are-lyophilized-oligos-stable-when-stored-frozen-The inherent chemical structure of RNA makes it less stable than DNA. RNases that degrade RNA are also more prevalent than DNases.
As RNA is a great deal more sensitive to degradation compared to DNA, for short term storage we recommend using...
http://eu.idtdna.com/pages/support/faqs/should-rna-be-stored-differently-than-dnaIDT ships gBlocks Gene Fragments dry unless otherwise requested. While dry, they should be stable up to two years.
After resuspend...
http://eu.idtdna.com/pages/support/faqs/what-are-optimum-storage-conditions-for-a-gblocks-gene-fragmentThe Alt-R CRISPR-Cas9 System provides custom CRISPR crRNA and a universal CRISPR tracrRNA, both of which are required for CRISPR-Cas9 genome editing using the Alt-...
http://eu.idtdna.com/pages/support/faqs/what-reagents-will-i-need-in-addition-to-the-alt-r-rnas-to-do-a-crispr-genome-editing-experimentThe kit does not include primers or PCR reagents for quantification of gene silencing. Separately, we offer primer sets for targeting human or mouse HPRT for use in SYBR® Green–based qPCR assays...
http://eu.idtdna.com/pages/support/faqs/do-the-positive-and-negative-controls-in-the-trifecta-kit-come-with-primers-and-rt-pcr-materials-