Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Can you ensure that there will be no incorrect bases at the 3' end position in my oligo?
IDT synthesizes oligos starting from the 3' end and successively adds bases toward the 5' end. The 3' end of your oligo will be the starting residue in the synthesis column and there is no possibility of incorrect bases being coupled to the support at the 3' end.