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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Can the PrimeTime® Gene Expression Master Mix be used in one-step, reverse-transcription qPCR?

The PrimeTime® Gene Expression Master Mix was developed for two-step, reverse-transcription qPCR. We do not recommend using the master mix in one-step reactions.