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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Are oligos synthesized by IDT deblocked or deprotected?

Yes. All protecting groups are removed from oligonucleotides after synthesis. The 5' and 3' ends of oligos carry a reactive hydryoxl group unless a modification is present. See the IDT Technical Report, "Chemical Synthesis of Oligonucleotides", for more information.

Custom DNA & RNA