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Your product is now available from Integrated DNA Technologies.
Many of the Swift products you have grown to love are now part of our new complete portfolio, xGen™ NGS. Through this new partnership we are pleased to offer you comprehensive next generation sequencing solutions.
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Welcome to the IDT family!
Find Archer now at IDT!
All Archer information is now available on IDT’s website. You can view Archer assays alongside IDT’s xGen™ NGS portfolio to find the best next generation sequencing solution for your lab.
Confidently detect more with Archer NGS assay solutions for your solid tumor, blood cancer, immune profiling, and genetic disease research.
Processing
An improved solution for generating large CRISPR HDR knock-ins
An improved solution for generating large CRISPR HDR knock-ins
CRISPR-based homology-directed repair (HDR) is an invaluable tool to facilitate specific mutations in a genomic region of interest in research studies. While many methods have been reported for improving HDR efficiency, achieving precise changes via HDR remains a challenge, particularly for large knock-ins. These insertions can be generated via HDR using enzymatically generated donor templates.
What you will learn:
- How dsDNA donor templates with novel end-modifications improve the frequency of HDR and reduce homology-independent (blunt) insertion events.
- How the addition of Alt-R HDR Enhancer V2, a small molecule compound, can further improve HDR rates.
- Design considerations when using large dsDNA templates for successful HDR experiments.
Published on: June 06, 2022