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qPCR probes—get the right scale at the right price

PrimeTime® Eco qPCR Probes provide a mid-range scale at a lower price per reaction

Only running 100–500 amplification reactions with FAM-labeled probes? Get a better price by using the PrimeTime Eco probes mid-range reaction scale for researchers. It's cheaper per reaction than larger reaction sizes and provides double-quenched FAM probes.

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IDT now offers FAM-labeled, double-quenched qPCR probes at a mid-range scale for researchers who need 100–500 amplification reactions, without having to pay for a much larger amount of probe. The PrimeTime Eco qPCR Probe is provided at 2.5 nmoles normalized yield, and is enough for approximately 500 amplifi­cation reactions.

PrimeTime Eco qPCR Probes use a FAM/ZEN™/IBFQ® dye/ quencher combination. These double-quenched probes include an internal quencher, the ZEN quencher, which provides greater background reduction and increased signal detection. For an example of how ZEN Double-Quenched Probes can improve real-time PCR data, see Figure 1, in the article, Two quenchers are better than one!

* IBFQ = Iowa Black Fluorescent Quencher

D3.4-FOF-EcoProbes Table1

Author(s)

Ellen Prediger, PhD, Senior Scientific Writer, IDT.

Published Oct 18, 2013

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Additional resources

Product focus

Free tools for qPCR & PCR design

Select primers and probes for your qPCR assays (human, mouse, rat), guaranteed at >90% efficiency.

Design primers or assays for PCR, qPCR, or sequencing with the PrimerQuest™ Tool.

Double-quenched probes

Probes designed with internal ZEN™ or TAO™ quencher, only 9 bases from the 5’ end.

Significantly decreases noise, while increasing sensitivity and precision.

PrimeTime Predesigned qPCR Assays

Probe-based and intercalating dye-based assays available. FAM-labeled, double-quenched probes.

For analysis of human, mouse, and rat transcriptomes.

PrimeTime Probe-based & Primer-only qPCR Assays

Assays offered in 3 versatile reaction scales—100, 500, or 2500. Tubes or 96-well plates.

Probes available with different dye–quencher combinations in the same plate.

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