IDT now offers FAM-labeled, double-quenched qPCR probes at a mid-range scale for researchers who need 100–500 amplification reactions, without having to pay for a much larger amount of probe. The PrimeTime Eco qPCR Probe is provided at 2.5 nmoles normalized yield, and is enough for approximately 500 amplification reactions.
PrimeTime Eco qPCR Probes use a FAM/ZEN™/IBFQ® dye/ quencher combination. These double-quenched probes include an internal quencher, the ZEN quencher, which provides greater background reduction and increased signal detection. For an example of how ZEN Double-Quenched Probes can improve real-time PCR data, see Figure 1, in the article, Two quenchers are better than one!
* IBFQ = Iowa Black Fluorescent Quencher