Performance data and guidance for better qPCR research
Real-time quantitative PCR (qPCR) is a mainstream method for accurate, sensitive, and fast gene expression analysis that is used in research and diagnostic applications. PrimeTime Gene Expression Master Mix for 5′ nuclease assays has been optimized for use with PrimeTime qPCR Assays—it also performs well with probe-based assays from other manufacturers. This versatile master mix is compatible with a wide range of instruments, with or without a reference dye, and can be used under standard or fast cycling conditions. PrimeTime master mix is extremely stable, delivering consistent qPCR performance in singleplex and multiplex reactions in overnight, high throughput experiments.
In the below webinar, High efficiency qPCR with the PrimeTime Gene Expression Master Mix from IDT, Dr Erik Wendlandt presents performance data, discusses key considerations for singleplex and multiplex experimental formats, and describes online tools that can help you plan your qPCR experiments. Access the recorded presentation below to learn about performing successful gene expression experiments with PrimeTime Gene Expression Master Mix. At the end of the recording, Dr Wendlandt answers questions received from webinar attendees.