Cold Spring Harbor Laboratory (CSHL) Genome Engineering CRISPR Frontiers

Confidence is the currency of science.

IDT will be participating in European Society for Human Genetics (ESHG) 2020 virtually this year. Learn why companies choose IDT and why IDT scores high in customer service—especially for our CRISPR products. The conference starts late morning EST on Wednesday, August 19 and ends late afternoon EST on Friday, August 21, 2020.

Poster presentations

• Characterization of synthetic single guide RNAs for CRISPR/Cas9 genome editing—An extensive evaluation of gRNA formats, purity, and delivery methods

  Presented by: Ashley Jacobi, Sr. Staff Scientist

• Large insertions mediated by CRISPR/Cas editing via improved HDR templates, and comprehensive characterization of editing events with long read sequencing.

  Presented by: Garrett Rettig, Director of Product Development

• High-throughput Off-Target Detection Supports Clinical Applications

  Presented by: Rolf Turk, Sr. Staff Scientist

• High-throughput analysis of repaired CRISPR double-stranded breaks reveals nuclease-specific complexity

  Presented by: Matt McNeill, Bioinformatics Sr. Staff Scientist

• Optimized fluorescent CRISPR enzyme fusions allow for enrichment of edited cells by fluorescence activated cell sorting

  Presented by: Steve Glen, Research Scientist

Workshop

Enhancing efficiency and specificity with improved CRISPR components and a new CRISPR analysis pipeline
Thursday, August 20, 2020
5:30 - 7:00 pm EST
Zoom link for workshop available upon registration

CRISPR genome editing in mammalian cells is challenging. Success hinges on efficiency and specificity—improving these metrics will save time and resources and enhance your success rate. Join us on August 20 to learn about our range of genome editing research projects, including our custom guide RNA and library capabilities, new custom-targeted nucleases, improved methods for mediating homology-directed repair (HDR), and a high throughput workflow for detecting off-target effects.

During the workshop, you will learn about:

• Our latest developments and customizations for guide RNAs, including pegRNAs and CRISPR libraries and our newest CRISPR nucleases with fluorescently labeled Cas9 variants.
• Improved design tools, enhancing reagents, and analysis tools used to efficiently generate SNPs and large knock-ins.
• Simplified methods for high-throughput detection and analysis of CRISPR-induced off-target effects, including a demonstration of our new, biologically informed CRISPR NGS analysis pipeline, CRISPAltRations.

Explore some of our CRISPR solutions

Overview
Superior Cas13 guide RNAs