{"id":897,"date":"2011-06-17T14:15:00","date_gmt":"2011-06-17T14:15:00","guid":{"rendered":"https:\/\/www.idtdna.com\/page\/modification-highlight-dithiol-modifier"},"modified":"2025-08-22T16:09:54","modified_gmt":"2025-08-22T16:09:54","slug":"modification-highlight-dithiol-modifier","status":"publish","type":"post","link":"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/","title":{"rendered":"Attach oligos to ligands or surfaces"},"content":{"rendered":"<h3>Quick Facts<\/h3>\n<p><strong>Availability:<\/strong> DNA or RNA location: 5&rsquo;, 3&rsquo;, or internally (shown) <strong>Scales: <\/strong>100 nmol to large scale <strong>Purification:<\/strong> HPLC required <strong>Symbol for ordering: <\/strong>\/5DTPA\/, \/iDTPA\/, \/3DTPA\/ <\/p>\n<p><figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/39865d15-3279-6e2e-aa53-ff00001c1b3c-cc_mod3.png\" alt=\"cc_mod3\" title=\"D-OLI11MH-dithiol-F1\" \/><figcaption class=\"image-caption\">Figure 1. Dithiol modifier.<\/figcaption><\/figure>\n<p> Thiol-modified oligonucleotides are used in attachment chemistry reactions to bind an oligo to a target. Targets are commonly gold, but can also include a variety of fluorescent and nonfluorescent moieties. The Dithiol modifier adds two functionalized thiol (SH) groups, increasing its target binding efficiency when compared to the conventional thiol modifier. An additional dithiol can be added for even greater binding efficiency [<a href=\"#references\">1<\/a>].<\/p>\n<p>Both Thiol- and Dithiol-modified oligonucleotides are shipped in their oxidized (disulfide) states and require reduction by dithiothreitol (DTT) or tris (2-carboxyethyl) phosphine (TCEP) prior to use.&nbsp;A protocol for the reduction of Thiol-modified oligonucleotides is available on the web site under SUPPORT &amp; EDUCATION &ndash; SUPPORT &ndash; User guides and protocols. Access it <a href=\"https:\/\/sfvideo.blob.core.windows.net\/sitefinity\/docs\/default-source\/protocol\/reduction-protocol-for-thiol-modified-oligonucleotides.pdf?sfvrsn=431c3407_8\">here<\/a>.<\/p>\n<p>To order, select dithiol from the 5&rsquo; mods, Internal mods, or 3&rsquo; mods tab on the oligo order<a href=\"\/site\/Catalog\/Modifications\/Category\/2\"> page<\/a>. Please note this modification requires <a href=\"\/pages\/products\/optional-services\/page-hplc-purification\">HPLC purification<\/a>.<\/p>\n<h3>Other modifications to facilitate your research<\/h3>\n<p>See what other modifications are available from IDT. You can find a list of standard modifications we make to oligonucleotides on the <a href=\"\/pages\/products\/custom-dna-rna\/oligo-modifications\">Modifications page <\/a>of our online catalog. And if you don&rsquo;t find what you are looking for, just send us a request. We often synthesize special, non-catalog requests. <a href=\"\/pages\/about\/contact-us\">Contact us<\/a>&nbsp;for more information.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Quick Facts Availability: DNA or RNA location: 5&rsquo;, 3&rsquo;, or internally (shown) Scales: 100 nmol to large scale Purification: HPLC required Symbol for ordering: \/5DTPA\/, \/iDTPA\/, \/3DTPA\/ Figure 1. Dithiol modifier. Thiol-modified oligonucleotides are used in attachment chemistry reactions to bind an oligo to a target. Targets are commonly gold, but can also include a [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"ct_builder_shortcodes":"","ct_template_type":"","ct_parent_template":0,"inline_featured_image":false,"footnotes":""},"class_list":["post-897","post","type-post","status-publish","format-standard","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Attach oligos to ligands or surfaces | IDT<\/title>\n<meta name=\"description\" content=\"Read how Thiol-modified oligonucleotides are used in attachment chemistry reactions to bind an oligo to a target.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Attach oligos to ligands or surfaces | IDT\" \/>\n<meta property=\"og:description\" content=\"Read how Thiol-modified oligonucleotides are used in attachment chemistry reactions to bind an oligo to a target.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/\" \/>\n<meta property=\"og:site_name\" content=\"IDT\" \/>\n<meta property=\"article:published_time\" content=\"2011-06-17T14:15:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-08-22T16:09:54+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/39865d15-3279-6e2e-aa53-ff00001c1b3c-cc_mod3.png\" \/>\n<meta name=\"author\" content=\"supreme_admin\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"supreme_admin\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/\"},\"author\":{\"name\":\"supreme_admin\",\"@id\":\"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/5259624c7d0e737830199587f61fd4c0\"},\"headline\":\"Attach oligos to ligands or surfaces\",\"datePublished\":\"2011-06-17T14:15:00+00:00\",\"dateModified\":\"2025-08-22T16:09:54+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/\"},\"wordCount\":241,\"commentCount\":0,\"image\":{\"@id\":\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/39865d15-3279-6e2e-aa53-ff00001c1b3c-cc_mod3.png\",\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/\",\"url\":\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/\",\"name\":\"Attach oligos to ligands or surfaces | IDT\",\"isPartOf\":{\"@id\":\"https:\/\/www.idtdna.com\/page\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/39865d15-3279-6e2e-aa53-ff00001c1b3c-cc_mod3.png\",\"datePublished\":\"2011-06-17T14:15:00+00:00\",\"dateModified\":\"2025-08-22T16:09:54+00:00\",\"author\":{\"@id\":\"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/5259624c7d0e737830199587f61fd4c0\"},\"description\":\"Read how Thiol-modified oligonucleotides are used in attachment chemistry reactions to bind an oligo to a target.\",\"breadcrumb\":{\"@id\":\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/#primaryimage\",\"url\":\"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/39865d15-3279-6e2e-aa53-ff00001c1b3c-cc_mod3.png\",\"contentUrl\":\"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/39865d15-3279-6e2e-aa53-ff00001c1b3c-cc_mod3.png\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.idtdna.com\/page\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Attach oligos to ligands or surfaces\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.idtdna.com\/page\/#website\",\"url\":\"https:\/\/www.idtdna.com\/page\/\",\"name\":\"Integrated DNA Technologies\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.idtdna.com\/page\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/5259624c7d0e737830199587f61fd4c0\",\"name\":\"supreme_admin\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/b33db2b8496475f7b5b0a33de06c5416bdad923515045a5e9106efc71f7916cc?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/b33db2b8496475f7b5b0a33de06c5416bdad923515045a5e9106efc71f7916cc?s=96&d=mm&r=g\",\"caption\":\"supreme_admin\"},\"sameAs\":[\"https:\/\/stage.idtdna.com\/page\"]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Attach oligos to ligands or surfaces | IDT","description":"Read how Thiol-modified oligonucleotides are used in attachment chemistry reactions to bind an oligo to a target.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier","og_locale":"en_US","og_type":"article","og_title":"Attach oligos to ligands or surfaces | IDT","og_description":"Read how Thiol-modified oligonucleotides are used in attachment chemistry reactions to bind an oligo to a target.","og_url":"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/","og_site_name":"IDT","article_published_time":"2011-06-17T14:15:00+00:00","article_modified_time":"2025-08-22T16:09:54+00:00","og_image":[{"url":"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/39865d15-3279-6e2e-aa53-ff00001c1b3c-cc_mod3.png","type":"","width":"","height":""}],"author":"supreme_admin","twitter_card":"summary_large_image","twitter_misc":{"Written by":"supreme_admin","Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/#article","isPartOf":{"@id":"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/"},"author":{"name":"supreme_admin","@id":"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/5259624c7d0e737830199587f61fd4c0"},"headline":"Attach oligos to ligands or surfaces","datePublished":"2011-06-17T14:15:00+00:00","dateModified":"2025-08-22T16:09:54+00:00","mainEntityOfPage":{"@id":"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/"},"wordCount":241,"commentCount":0,"image":{"@id":"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/#primaryimage"},"thumbnailUrl":"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/39865d15-3279-6e2e-aa53-ff00001c1b3c-cc_mod3.png","inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/","url":"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/","name":"Attach oligos to ligands or surfaces | IDT","isPartOf":{"@id":"https:\/\/www.idtdna.com\/page\/#website"},"primaryImageOfPage":{"@id":"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/#primaryimage"},"image":{"@id":"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/#primaryimage"},"thumbnailUrl":"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/39865d15-3279-6e2e-aa53-ff00001c1b3c-cc_mod3.png","datePublished":"2011-06-17T14:15:00+00:00","dateModified":"2025-08-22T16:09:54+00:00","author":{"@id":"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/5259624c7d0e737830199587f61fd4c0"},"description":"Read how Thiol-modified oligonucleotides are used in attachment chemistry reactions to bind an oligo to a target.","breadcrumb":{"@id":"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/#primaryimage","url":"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/39865d15-3279-6e2e-aa53-ff00001c1b3c-cc_mod3.png","contentUrl":"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/39865d15-3279-6e2e-aa53-ff00001c1b3c-cc_mod3.png"},{"@type":"BreadcrumbList","@id":"https:\/\/eu.idtdna.com\/page\/support-and-education\/decoded-plus\/modification-highlight-dithiol-modifier\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.idtdna.com\/page\/"},{"@type":"ListItem","position":2,"name":"Attach oligos to ligands or surfaces"}]},{"@type":"WebSite","@id":"https:\/\/www.idtdna.com\/page\/#website","url":"https:\/\/www.idtdna.com\/page\/","name":"Integrated DNA Technologies","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.idtdna.com\/page\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/5259624c7d0e737830199587f61fd4c0","name":"supreme_admin","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.idtdna.com\/page\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/b33db2b8496475f7b5b0a33de06c5416bdad923515045a5e9106efc71f7916cc?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/b33db2b8496475f7b5b0a33de06c5416bdad923515045a5e9106efc71f7916cc?s=96&d=mm&r=g","caption":"supreme_admin"},"sameAs":["https:\/\/stage.idtdna.com\/page"]}]}},"_links":{"self":[{"href":"https:\/\/eu.idtdna.com\/page\/wp-json\/wp\/v2\/posts\/897","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/eu.idtdna.com\/page\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/eu.idtdna.com\/page\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/eu.idtdna.com\/page\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/eu.idtdna.com\/page\/wp-json\/wp\/v2\/comments?post=897"}],"version-history":[{"count":1,"href":"https:\/\/eu.idtdna.com\/page\/wp-json\/wp\/v2\/posts\/897\/revisions"}],"predecessor-version":[{"id":960,"href":"https:\/\/eu.idtdna.com\/page\/wp-json\/wp\/v2\/posts\/897\/revisions\/960"}],"wp:attachment":[{"href":"https:\/\/eu.idtdna.com\/page\/wp-json\/wp\/v2\/media?parent=897"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}