Click the highlighted boxes at the left that represent your start and stop codons.
If you would like to designate a specific region to optimize, or if your start or stop codon is not shown, use the "Manual" option above.
Use the mouse to highlight the region to optimize. Click the button below to set reading frame.
Enter the first and last nucleotide base of your reading frame (inclusive). The first and last codons will highlight at the left.
To optimze a portion of the sequence, enter start and end locations after the sequence. Start and end locations are inclusive.
To optimize the whole sequence, leave the start and end locations blank.
Reading frame selection not available for FASTA entry. Amino acid sequences are entered using single-letter abbreviations.