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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

I transfected the cells with Alt-R™ S.p. Cas9-GFP. When should I sort the cells by fluorescence activated cell sorting (FACS) to enrich for transfected cells?

Our internal research data have shown that sorting cells within 24 hours after transfection of RNP complexes containing Alt-R S.p. Cas9-GFP nuclease gives detectable signal.