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xGen™ Human Mitochondrial DNA (mtDNA) Hybridization Panel

Complete coverage of the human mitochondrial genome

The xGen Human Mitochondrial DNA (mtDNA) Hyb Panel is designed to simplify the investigation of human mitochondrial genes by delivering optimal coverage across the entire >16 kb human mitochondrial genome in research samples.

xGen NGS—made for human mtDNA research.

Ordering

  • Identify important variants in research samples with complete coverage of the human mitochondrial genome
  • Easily expand your target space with a predesigned spike-in panel that can be used together with other target enrichment panels
  • Consistently achieve reliable results with individually synthesized probes

For the xGen Hybridization and Wash Kit, xGen Universal Blockers, xGen Library Amplification Primer Mix, and/or xGen Human Cot DNA, please visit the xGen Hybridization Capture Core Reagents page.

Product details

The xGen Human Mitochondrial DNA (mtDNA) Hyb Panel provides coverage of the human mitochondrial genome in research studies. The panel consists of 138 individually-synthesized xGen Hyb Probes and delivers reliable coverage of the entire >16 kb mitochondrial genome.

This flexible, modular panel can be used as either a standalone† or spike-in panel, for cost-effective sequencing of variants in mitochondrial DNA in research samples. When added to other xGen Hyb Panels, the xGen Human mtDNA Hyb Panel does not affect the high target specificity or coverage uniformity of the original panel.

†Because the mitochondrial genome comprises a small target space, the xGen Human mtDNA Hyb Panel contains relatively few probes compared to other target capture panels. When used as a standalone panel, expect lower on-target results than when used as a spike-in to a larger panel. This is typical of small panels in hybridization capture enrichment.

Product data

Efficient capture with combined panels

Figure 1. An example showing the spike in of the xGen Human mtDNA Hyb Panel does not impact on-target rate of recipient panel. DNA libraries were prepared from 100 ng of NA12878 genomic DNA (Coriell). 500 ng of the libraries were enriched with a custom panel comprised of 509 xGen Hyb Probes, with (n=8) and without spike in (n=3) of the xGen mtDNA Hyb Panel. The enriched libraries were sequenced on a MiSeq® System (Illumina) using 2 x 150 paired-end reads. Each sample was sub-sampled to 0.5M total reads. The averages of on-target values (with 150 bp flank) across experiments are shown.

Uniform coverage of targeted regions

Figure 2. Example of uniform coverage of targeted regions using xGen Human mtDNA Hyb Panel. Genomic DNA libraries were enriched using the human mtDNA Panel spiked into a custom panel containing 509 probes (n=8). Enriched samples were sequenced on a MiSeq system using 2 x 150 paired-end reads. Total reads for each sample were sub-sampled to 0.5M total reads. The average normalized target coverage ranged between 0.56X and 1.31X for the regions targeted by the mtDNA Panel. Normalized target coverage bounds at 0.5X and 1.5X are denoted by the doted lines.

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