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Precise genome editing with A.s. Cas12a (Cpf1) Ultra: A mutant protein with the reliability of S.p. Cas9

IDT staff scientist Dr Chris Vakulskas will talk about the development of a mutant protein, A.s. Cas12a (Cpf1) Ultra, which has dramatically improved cleavage to a level that rivals S.p. Cas9 without altering its PAM specificity.

The A.s. Cas12a (Cpf1) Ultra protein also demonstrates low-temperature cleavage activity superior to that of L.b. Cas12a, making it a universal Cas12a solution for genome editing in both animal and plant species.

Finally, IDT research scientist Bernice Thommandru will describe optimal HDR methods and reagents for maximizing HDR rates when using either A.s. Cas12a (Cpf1) Ultra or S.p. Cas9.

Published on: June 20, 2019

Categories:

Gene knockout Gene mutation Genome editing Homology-directed repair CRISPR genome editing Alt-R CRISPR-Cas9 Alt-R CRISPR-Cpf1 Alt-R CRISPR nucleases Alt-R HDR Donor Oligos Cancer Research Clinical diagnostics CRISPR diagnostics Webinar CRISPR workflow CRISPR-Cas systems Alt-R enzymes Alt-R CRISPR-Cas12a Alt-R CRISPR-Cas9 Alt-R other enzymes Detection of CRISPR edits

Tags:

crispr cas9 Cpf1 PAM genome editing CRISPR-Cas9 CRISPR genome editing homology directed repair guide RNA cas12a