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CRISPR knock-in: Tips and tricks

What is needed for a successful CRISPR knock-in experiment? Since CRISPR knock-ins have intrinsically low efficiency, it is important to carefully select all the components of a knock-in experiment and its conditions. For a successful knock-in, it is recommended to choose the right guide RNA, DNA donor template, and Cas9 nuclease, as well as consider using silent mutations to avoid premature dsDNA degradation and recutting of the target DNA.

Published on: November 28, 2023