Pipet Tips
Ideas to Streamline Your Research

Do Your qPCR Assays Come With Sequence Information? They Should. Here Is Why.

qPCR assays (primer & probe sets) from other suppliers are often provided without sequence information. IDT always gives you the sequences to the oligos you order. And that can be very important. Read why.

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Recommended Dye Combinations for Multiplex qPCR

Recommendations for selecting dyes for multiplex qPCR that minimize background and avoid overlap of fluorescent signals. Included is a table of compatible dyes for multiplexing on common qPCR instruments and a list of suggested quenchers.

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My Oligos Have Arrived; Now What?

Recommendations for resuspension and storage of newly received oligonucleotides.

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Designing PCR Primers and Probes

General guidelines for designing primers and probes and for choosing target locations for PCR amplification.

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Calculations: Converting from Nanograms to Copy Number

Calculation often used when creating a qPCR standard curve. Link to free, online tool that will do it for you.

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GAPDH, a Good Reference Sequence?

Housekeeping genes commonly used as internal controls, often may not be the best choices due to lesser known functions and the presence of pseudogenes, some of which, in the case of GAPDH---used here as an example--may be expressed.

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Easily-Designed Standard Curves for qPCR

An easy way to combine control templates/multiple targets onto a single construct and the advantages that can provide for PCR experiments.

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Which Type of Purification Should I Choose?

Recommendations for oligonucleotide purification based on oligo length, application, yield required, and presence of modifications.

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Deletions During Cloning

Explanations for why sequenced clones sometimes show deletions and some suggestions for mitigating this result.

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Annealing Oligonucleotides

Tips on making double-stranded DNA from single-stranded, complementary oligonucleotides.

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Calculation Tips for Resuspending and Diluting Nucleic Acids

Easy guidelines for making a 100 uM solution; calculating nmoles, ug, copy number, and concentration; and determining concentration equivalencies

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Sample Preparation for Successful qPCR

Considerations for qPCR sample preparation for obtaining accurate and consistent results.

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Steps for a Successful qPCR Experiment

This article will focus on 5′ nuclease assay design and experimental setup considerations that will assist in obtaining accurate and consistent results.

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Running Agarose and Polyacrylamide Gels

Electrophoresis with agarose and polyacrylamide gels is one of the most widely used tools in molecular biology. Gels provide a simple, low-cost way to separate nucleic acids based on size for quantification and purification.

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Tips for Using BLAST to Locate PCR Primers

Need a quick way to find the location of primers within a gene or the expected size of the resultant PCR product? In this tip we show you how to get this information using BLAST.

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