Core Concepts
Scientific Fundamentals Explained

Interpreting Melt Curves: An Indicator, Not a Diagnosis

Examining PCR melt curve data to determine what it can/cannot tell us about resulting PCR amplicons.

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Target Enrichment Facilitates Focused Next Generation Sequencing

The rationale and benefits of enriching subsets of the genome (target enrichment by hybrid capture) prior to sequencing.

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Modification Highlight: Modifications that Block Nuclease Degradation

Description of modifications that can be added to an oligo to limit nuclease degradation, for example, when experiments where the oligos are to be used in cell culture or in vivo.

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CRISPR and Cas9 for Flexible Genome Editing

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) are sequences that serve as an adaptive defense ("immune") systems in bacteria and archaea . In conjunction with CRISPR associated (Cas) proteins, CRISPR sequences are able to recognize and cleave complementary DNA sequences. This natural mechanism has been coopted by scientists for targeted gene editing or removal. This article describes some of the early applications for which this technology is being used.

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Digital PCR (dPCR)—What Is It and Why Use It?

General overview of dPCR and how it can be used for qPCR applications, including multiplex qPCR.

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