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RxnReady™ Primer Pools
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Base Notations
- Standard Bases, (A, C, G, T) are not case sensitive.
- Mixed Bases, expressed in UPPERCASE.
- RNA, expressed as 'r_', for example rA, rU.
- 2' O-methyl RNA bases are entered as 'm_'.
- Phosphorothioated DNA bases are entered as '_*'
- Phosphorothioated RNA bases are entered as 'r_*'.
- Phosphorothioated 2'-O-methyl RNA bases are entered as 'm_*'.
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Melting Temperature Settings
| Parameter sets | |
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| Target type | |
| Oligo Conc | µM |
| Na+ Conc | mM |
| Mg++ Conc | mM |
| dNTPs Conc | mM |
+32 (0) 16 28 22 60
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